cultivo in vitro do arroz (Oryza sativa L.): conceitos básicos e protocolo
Resumo
O arroz, além de sua importância econômica no Brasil e no mundo, destaca-se também por ser planta modelo para estudos moleculares da família Poaceae. O seu cultivo in vitro para produção de embriões somáticos e regeneração de plantas é uma ferramenta valiosa para os programas de melhoramento genético do arroz, podendo auxiliar no aumento da variabilidade genética. Sua aplicação também se estende aos estudos de caracterização funcional de genes provenientes de estudos de genômica funcional. Portanto, um sistema de cultivo in vitro eficiente, meios otimizados e genótipos adequados são de extrema importância.
Nesse sentido, é que o documento ora apresentado vem preencher uma lacuna nesse assunto, pois poucas são as informações sobre a cultura de tecidos de arroz, em português. Com esta publicação, abordam-se os conceitos básicos da teoria e da prática dessa atividade, e adicionalmente descrevem-se cada etapa de dois protocolos de indução e regeneração de duas cultivares de arroz.
Palavras-chave
Referências
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